Name |
Active human USP14 full length protein
|
Supplier |
Abcam
|
Catalog |
ab188696
|
Category |
Protein
|
Prices |
$232.00
|
Sizes |
25 µg
|
Applications |
FA SDS-PAGE
|
Species Reactivities |
Human
|
Nature |
Recombinant
|
Source |
E. coli
|
Purity |
>95% by SDS-PAGE . Purified under reducing conditions and visualized by Coomassie Blue.
|
Bioactivity |
The activity of ab188696 was measured at 2.0 RFU/min/μg using an enzyme concentration of 18 μM and a Ub-AMC concentration of 4 μM in 100 mM HEPES, pH 8.5, 10 mM DTT, and 5% Glycerol at 25°C. USP14 activity is greatly stimulated in the presence of 19S proteasome.
|
SwissProt/Accession |
P54578
|
Gene |
USP14
|
Residue |
1 to 494
|
Sequence |
MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKG GTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASA MELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQ YITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDA NECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVE FETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITK QSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFP LMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVK YEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKF DDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ
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