All lanes : Anti-SPON1 antibody ( ab40797 ) at 2 µg/ml Lane 1 : Brain (Rat) Tissue Lysate Lane 2 : Spinal Cord (Rat) Tissue Lysate Lane 3 : Ovary (Mouse) Tissue Lysate Lane 4 : Mouse (14 day old) Fetal Tissue Lysate Lane 5 : Brain (Rat) Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/ml Lane 6 : Spinal Cord (Rat) Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/ml Lane 7 : Ovary (Mouse) Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/ml Lane 8 : Mouse (14 day old) Fetal Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/ml Lysates/proteins at 15 µg per lane. developed using the ECL technique Performed under reducing conditions. Predicted band size : 91 kDa Observed band size : 91 kDa