Active human Visfatin full length protein
| Name | Active human Visfatin full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198458 |
| Category | Protein |
| Prices | $1,004.00 |
| Sizes | 50 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | 87 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥0.6 pmol/min/µg Assay Conditions: The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl 2 , 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 µg/ml of alcohol dehydrogenase, 10 µg/ml of NMNAT, 1.5% alcohol, 1 mM DTT, 0.02% BSA, and various amount of ab198458 in a final volume of 50 µl. Reactions were initiated with the addition of ab198458, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was continuously monitored for 30 min at room temperature. |
| SwissProt/Accession | P43490 |
| Gene | NAMPT |
| Residue | 2 to 491 |
| Sequence | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSNPAAEAEFNILLATDSYKV THYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYL KGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVP EGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKK ILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDT VAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFS SVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDT VLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQ KMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVAD PNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKS YSFDEIRKNAQLNIELEAAHH |
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