All lanes : Anti-Superoxide Dismutase 1 antibody ( ab45777 ) at 0.5 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab7909 ) Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate ( ab7902 ) Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate ( ab7900 ) Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Superoxide Dismutase 1 peptide (ab46993) at 1 µg Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) with Human Superoxide Dismutase 1 peptide (ab46993) at 1 µg/ml Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab7909 ) with Human