Recombinant BAZ1B (1340-1457) protein

• Supplier: ActiveMotif
• Catalog: 31444
• Category: Protein
• Prices: $395.00, $2,600.00
• Sizes: 100 μg, 1 mg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: BAZ1B
• Description: The protein corresponding to amino acids 1340-1457 that contains the bromodomain sequence of human BAZ1B protein (accession number NP_115784.1) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with a molecular weight of 20.2 kDa

Product images

Recombinant BAZ1B (1340-1457) protein gel. BAZ1B (1340-1457) protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Recombinant BAZ1B (1340-1457) activity using AlphaScreen. BAZ1B (1340-1457) titration was assessed using an AlphaScreen® assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity. 3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with BAZ1B (1340-1457) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.