Recombinant MLL3 Complex protein gel. Recombinant MLL3 Complex run on an SDS-PAGE gel and stained with Coomassie blue.
Recombinant MLL3 Complex activity using AlphaLISA. MLL3 Complex was used in an AlphaLISA assay to determine enzyme linearity. This data was generated and kindly provided courtesy of ChemPartner.
Recombinant MLL3 Complex activity assay. 3. 3 µM H3K4me0 peptide was incubated with 10 nM MLL3 Complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3. 3 µM H3K4me0 peptide was used as negative control. Catalytic Ability: ~165 turnovers/ enzyme molecule