Recombinant SMARCA2 / BRM (1367-1511) protein

• Supplier: ActiveMotif
• Catalog: 31449
• Category: Protein
• Prices: $395.00
• Sizes: 100 μg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: SMARCA2
• Description: The peptide corresponding to amino acids 1367-1511 that contains the bromodomain sequences of SMARCA2 / BRM (accession number NP_003061.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 22.96 kDa

Product images

Recombinant SMARCA2 / BRM (1367-1511) protein gel. SMARCA2 / BRM (1367-1511) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.

Recombinant SMARCA2 / BRM (1367-1511) activity using AlphaScreen. SMARCA2 / BRM (1367-1511) titration and inhibition were assessed using an AlphaScreen® assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. An IC50 dose response assessment of reference compound PFI-3 is also shown. This data was generated and kindly provided courtesy of ChemPartner.

HTRF Assay for Recombinant SMARCA2 / BRM (1367-1511) activity. 3. 3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with SMARCCA2/BRM (1367-1511) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.