Recombinant SMARCA4 / BRG1 (1448-1569) protein
| Name | Recombinant SMARCA4 / BRG1 (1448-1569) protein |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31401 |
| Category | Protein |
| Prices | $395.00 |
| Sizes | 100 μg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Gene | SMARCA4 |
| Description | The peptide corresponding to amino acids 1448-1569 that contains the bromodomain sequences of SMARCA4 (accession number NP_003063.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 20.4 kDa |
| Supplier Page | Shop |
Product images
Recombinant SMARCA4 / BRG1 (1448-1569) protein gel. SMARCA4 / BRG1 (1448-1569) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
HTRF Assay for Recombinant SMARCA4/BRG1 (1448-1569) Activity. 3. 3 µM histone peptide H3K14ac was incubated with SMARCA4/BRG1 (1448-1569) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.
HTRF Assay for Recombinant SMARCA4/BRG1 (1448-1569) Activity. 3. 3 µM histone peptide H3K14ac was incubated with SMARCA4/BRG1 (1448-1569) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.