Recombinant TRIM24 (862-980) protein gel. TRIM24 (862-980) protein was run on an SDS-PAGE gel and stained with Coomassie blue.
HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 µM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 µM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.