Recombinant JARID1C / KDM5C protein gel. JARID1C / KDM5C protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
JARID1C / KDM5C activity assay. Recombinant JARID1C / KDM5C activity measured using a demethylation assay. MALDI-TOF was used for detection.
JARID1C / KDM5C activity assay. 3. 3 µM H3K4me3 peptide was incubated with JARID1C / KDM5C in reaction buffer for 1 hour at room temperature. JARID1C / KDM5C enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.