Recombinant JMJD2A / KDM4A protein gel. JMJD2A / KDM4A protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
JMJD2A / KDM4A activity assay. Recombinant JMJD2A / KDM4A activity measured using a demethylation assay. MALDI-TOF was used for detection.
JMJD2A / KDM4A activity assay. 3. 3 µM H3K9me3 peptide was incubated with 60 nM JMJD2A / KDM4A in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3. 3 µM H3K9me3 peptide was used as negative control.