Recombinant PHF8 protein gel. PHF8 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
PHF8 activity assay. Recombinant PHF8 activity measured using a demethylation assay. MALDI-TOF was used for detection.
Recombinant PHF8 protein activity assay. 3. 3 μM H3K9me2 peptide was incubated with PHF8 protein in reaction buffer for 1 hours at room temperature. PHF8 enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody.