Recombinant SETDB1 protein
| Name | Recombinant SETDB1 protein |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31452 |
| Category | Protein |
| Prices | $425.00 |
| Sizes | 20 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus |
| Gene | SETDB1 |
| Description | Recombinant SETDB1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001138887.1) with an N-terminal FLAG tag |
| Supplier Page | Shop |
Product images
Recombinant SETDB1 protein gel. SETDB1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.
SETDB1 activity assay. HMT assay measuring the activity of Recombinant SETDB1. Activity was detected by fluorography.
HTRF assay for Recombinant SETDB1 protein activity . 3 µM histone peptide H3 (1-21) was incubated with SETDB1 in reaction buffer including 50 mM Tris-HCl pH 8. 6, 0. 02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 µM SAM for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction product.