Recombinant UTX / KDM6A protein gel. UTX / KDM6A protein was run on an SDS-PAGE gel and stained with Coomassie blue.
UTX / KDM6A activity assay. Recombinant UTX / KDM6A activity measured using a demethylation assay. MALDI-TOF was used for detection.
UTX / KDM6A AlphaLISA assay. UTX / KDM6A enzyme was used in an AlphaLISA assay to determine enzyme linearity, 2OG Km and IC50 of two reference compounds. 10 nM-20 nM KDM6A was used to develop an HTS screening assay and well-to-well reproducibility was also assessed at Z’=0. 64. Demethylated peptide (H3K27me2) was measured using methylation-specific antibody. This data was generated and kindly provided courtesy of ChemPartner.