Active mouse PDE7A protein fragment
| Name | Active mouse PDE7A protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab198756 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 10 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Mouse |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | > 22 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥120 pmol/min/µg. Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C. Assayed using a PDE7A kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198756 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
| SwissProt/Accession | Q13946 |
| Gene | PDE7A |
| Residue | 147 to 482 |
| Sequence | MLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMVKLRR FLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASSVTPWDILLSL IAAATHDLDHPGVNQPFLIKTNHYLATLYKNSSVLENHHWRSAVGLLRES GLFSHLPLESRQEMEAQIGALILATDISRQNEYLSLFRSHLDKGDLHLDD GRHRHLVLQMALKCADICNPCRNWELSKQWSEKVTEEFFHQGDIEKKYHL GVSPLCDRQTESIANIQIGFMTYLVEPLFTEWARFSDTRLSQTMLGHVGL NKASWKGLQRQQPSSEDANAAFELNSQLLTQENRLS |
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