Active mouse PDE7A protein fragment

Name Active mouse PDE7A protein fragment
Supplier Abcam
Catalog ab198756
Category Protein
Prices $1,250.00
Sizes 10 µg
Applications FA SDS-PAGE
Species Reactivities Mouse
Nature Recombinant
Source Baculovirus infected sf9 cells
Tag/Conjugation GST tag N-Terminus
Purity > 22 % by SDS-PAGE.
Bioactivity Specific Activity: ≥120 pmol/min/µg. Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C. Assayed using a PDE7A kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198756 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
SwissProt/Accession Q13946
Gene PDE7A
Residue 147 to 482
Sequence MLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMVKLRR FLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASSVTPWDILLSL IAAATHDLDHPGVNQPFLIKTNHYLATLYKNSSVLENHHWRSAVGLLRES GLFSHLPLESRQEMEAQIGALILATDISRQNEYLSLFRSHLDKGDLHLDD GRHRHLVLQMALKCADICNPCRNWELSKQWSEKVTEEFFHQGDIEKKYHL GVSPLCDRQTESIANIQIGFMTYLVEPLFTEWARFSDTRLSQTMLGHVGL NKASWKGLQRQQPSSEDANAAFELNSQLLTQENRLS
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