Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5µg/ml mDLL1-Fc (ab108549) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
Induction of Hes-1 with the treatment of recombinant mouse DLL1-Fc (ab108549). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc (ab108549) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH. Lane 1: ab108549, 0 min Lane 2: ab108549, 10 min Lane 3: ab108549, 30 min Lane 4: ab108549, 1 hr Lane 5: ab108549, 2 hr Lane 6: ab108549, 4 hr Lane 7: ab108549, 8 hr Lane 8: ab108549, 24 hr
Adipogenesis inhibition. Macroscopic pictures.