All lanes : Anti-Doublecortin (phospho S28) antibody - Neuronal Marker ( ab23544 ) at 1 µg/ml Lane 1 : Mouse Adult Brain Whole Tissue Lysate Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old ( ab7188 ) Lane 3 : Mouse Adult Brain Whole Tissue Lysate with Mouse Doublecortin (phospho S28) peptide ( ab31511 ) at 1 µg/ml Lane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old ( ab7188 ) with Mouse Doublecortin (phospho S28) peptide ( ab31511 ) at 1 µg/ml Lane 5 : Mouse Adult Brain Whole Tissue Lysate with Mouse Doublecortin peptide (ab31512) at 1 µg/ml Lane 6 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old ( ab7188 ) with Mouse Doublecortin peptide (ab31512) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 50 kDa Additional bands at : 35 kDa (possible degradation product).