All lanes : Anti-NMDAR2A (phospho Y1325) antibody ( ab16646 ) at 1 µg/ml Lane 1 : mouse brain lysate Lane 2 : mouse brain lysate with Mouse NMDAR2A (phospho Y1325) peptide (ab16985) at 1 µg/ml Lane 3 : mouse brain lysate with Mouse NMDAR2A peptide ( ab16990 ) at 1 µg/ml Lysates/proteins at 20 µg per lane. Performed under reducing conditions. Predicted band size : 163 kDa Observed band size : 163 kDa Additional bands at : 50 kDa (possible non-specific secondary antibody binding),~130 kDa,~150 kDa. We are unsure as to the identity of these extra bands. Exposure time : 5 minutes Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom