Recombinant Human CUL3/RBX1

• Supplier: Creative Biomart
• Catalog: CUL3-148H
• Category: Protein
• Species Reactivities: Human
• Nature: Recombinant
• Source: Insect cells
• Bioactivity: E3 Ligase Assay: The activity of Cul3/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul3/Rbx1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL2 at 4?C was compared at two time points T0 and T2 minutes. Neddylation of the Cul3 subunit in the presence of His-DCNL2 was demonstrated.
• SwissProt/Accession: Q13618
• Gene: CUL3
• Description: Recombinant human CUL3 (amino acid residues 1-768), RBX1 (amino acid residues 1-108) was expressed in Insect (Sf21) cells.