All lanes : Anti-YB1 antibody ( ab12148 ) at 1.4 µg/ml Lane 1 : HeLa Nuclear lysate Lane 2 : HeLa Whole cell lysate Lane 3 : MCF-7 cell lysate Lane 4 : Jurkat whole cell lysate Lane 5 : HEK293 Whole cell lysate Lane 6 : HeLa Nuclear lysate with YB1 peptide (ab12411) at 1 µg/ml Lane 7 : HeLa Whole cell lysate with YB1 peptide (ab12411) at 1 µg/ml Lane 8 : MCF-7 cell lysate with YB1 peptide (ab12411) at 1 µg/ml Lane 9 : Jurkat whole cell lysate with YB1 peptide (ab12411) at 1 µg/ml Lane 10 : HEK293 whole cell lysate with YB1 peptide (ab12411) at 1 µg/ml Lysates/proteins at 20 µg per lane. Predicted band size : 36 kDa Observed band size : 36 kDa Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands. A band of approx 50 KDa was partially blocked in several cell lines using a YB1 blocking peptide (ab12411). This suggests the antibody recognises YB1. Another band of approx 36 kDa also appears to be recognised, which may be a cleavage product of YB1.