Active rat PDE4D full length protein
| Name | Active rat PDE4D full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198442 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 5 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Rat |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >= 80 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥ 22,535 pmole/min/µg. Assay Description: One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of rPDE4D at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
| SwissProt/Accession | Q08499 |
| Gene | PDE4D |
| Residue | 2 to 803 |
| Sequence | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDPLVPRGSPGEFEAEGSSVPARAGSDEGSDS SGGAALKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQLQ PPPPPPLPPPPPPPGATRGRYASSGASRVRHRGYSDTERYLYCRAMDRTS YAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSG LILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTP FAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQK LASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRS GNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSS LTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMH TIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTH VLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMY NDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDM SKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPT KPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFI DYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQED GRQGQTEKFQFELTLEEDGESDTEKDSGSQV |
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