Active T4 DNA Ligase full length protein

• Supplier: Abcam
• Catalog: ab68649
• Category: Protein
• Prices: $279.00
• Sizes: 20000 units
• Applications: FA
• Nature: Recombinant
• Source: E. coli
• Bioactivity: 167,000 U/ml. Unit Defenition: One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA (5´ DNA termini concentration of 0.12 µM, 300µg/ml) in a total reaction volume of 20 µl in 30 minutes at 16 ° C in 1X T4 DNA Ligase Reaction Buffer: (50mM Tris-HCl (pH 7.8 at 25°C), 10mM Magnesium chloride, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 µM in 5´ termini)).
• Gene: LIG4