| Name | Recombinant Human UBA7, His-tagged |
|---|---|
| Supplier | Creative Biomart |
| Catalog | UBA7-171H |
| Category | Protein |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Insect cells |
| Tag/Conjugation | His |
| Purity | >98% by SDS-PAGE |
| Bioactivity | E1 Thioester ISG15 Loading Assay: The activity of His-UBA7 was validated by loading ISG15 onto the active cysteine of His-UBA7. Incubation of the His-UBA7 enzyme in the presence of ISG15 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the ISG15/His-UBA7 thioester bond to the reducing agent DTT was confirmed. |
| SwissProt/Accession | P41226 |
| Gene | UBA7 |
| Description | Recombinant human UBA7 (amino acid residues 1-1012), fused with N-terminal His, was expressed in Insect sf21. |
| Supplier Page | Shop |