All lanes : rabbit polyclonal antibody anti AMARV GP at 0.5 µg/ml Lane 1 : AMARV GP protein fragment (ab190126) at 0.1 µg Lane 2 : AMARV GP protein fragment (ab190126) at 0.5 µg Lane 3 : AMARV GP protein fragment (ab190126) at 1 µg Secondary anti-rabbit IgG-HRP conjugate, followed by substrate. Predicted band size : 74 kDa
SDS-PAGE analysis of 1 μg and 5 μg (lanes 1, 2 respectively) of ab190126 under denaturing and reducing conditions.
Plate was coated with ab190126 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TM substrate. OD650 is reported. Background of ab190126 coated plate without positive control serum was 0.051 OD650.