| Name | Active human AMSH full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab188715 |
| Category | Protein |
| Prices | $572.00 |
| Sizes | 50 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected Sf21 cells |
| Tag/Conjugation | proprietary tag N-Terminus |
| Purity | > 85 % by SDS-PAGE. |
| Bioactivity | Binds STAM protein with Kd = ~7 µM. In in vitro assays containing 200 nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM. Half-maximal increase occurred at 0.4 µM STAM. Typical enzyme concentration for use in vitro ranges from 100-500 nM depending on conditions and substrate. Suggested Reaction Buffer for plate-based assays is 50 mM Hepes, pH 8.0, 100 mM NaCl, and 0.1 mg/ml BSA. AMSH activity is partially or fully inhibited by DTT at concentrations above 1 mM. |
| SwissProt/Accession | O95630 |
| Gene | STAMBP |
| Residue | 1 to 424 |
| Sequence | MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIY SEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAF PKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVA QQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDL EKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPT IDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVL IPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLH THCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSK DPPLFCSCSHVTVVDRAVTITDLR |
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