![Western Blot: p75NTR/NGF Receptor Protein [NBP1-46099] - A sample of NGF R (209 aa) - Fc Chimera without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 ug protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of NGF R (209 aa) - Fc Chimera. Spots within the spot train were cut from the gel and identified as NGF R (209 aa) - Fc Chimera by protein mass fingerprinting.](http://www.bioprodhub.com/system/product_images/pp_products/5/sub_1/200_p75NTR-NGF%2520Receptor%2520Protein-Western%2520Blot-NBP1-46099-img0007.jpg)
Western Blot: p75NTR/NGF Receptor Protein [NBP1-46099] - A sample of NGF R (209 aa) - Fc Chimera without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 ug protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of NGF R (209 aa) - Fc Chimera. Spots within the spot train were cut from the gel and identified as NGF R (209 aa) - Fc Chimera by protein mass fingerprinting.
![Western Blot: p75NTR/NGF Receptor Protein [NBP1-46099] - Lane 1 - MW markers; Lane 2 - NGF R (209 aa) - Fc Chimera; Lane 3 - NGF R (209 aa) - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4 - NGF R (209 aa) - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 ug protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates the presence of O-linked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.](http://www.bioprodhub.com/system/product_images/pp_products/5/sub_1/201_p75NTR-NGF%2520Receptor%2520Protein-Western%2520Blot-NBP1-46099-img0006.jpg)
Western Blot: p75NTR/NGF Receptor Protein [NBP1-46099] - Lane 1 - MW markers; Lane 2 - NGF R (209 aa) - Fc Chimera; Lane 3 - NGF R (209 aa) - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4 - NGF R (209 aa) - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 ug protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates the presence of O-linked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.
![SDS-Page: p75NTR/NGF Receptor Protein [NBP1-46099] - Post-translational modifications result in protein heterogeneity. The densitometry scan, derived from the 2D gel image above, demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. Triangle indicates theoretical pI and MW of the protein.](http://www.bioprodhub.com/system/product_images/pp_products/5/sub_1/202_p75NTR-NGF%2520Receptor%2520Protein-SDS-Page-NBP1-46099-img0003.jpg)
SDS-Page: p75NTR/NGF Receptor Protein [NBP1-46099] - Post-translational modifications result in protein heterogeneity. The densitometry scan, derived from the 2D gel image above, demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. Triangle indicates theoretical pI and MW of the protein.