Active human CERK full length protein
| Name | Active human CERK full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab196089 |
| Category | Protein |
| Prices | $858.00 |
| Sizes | 10 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag C-Terminus |
| Purity | >= 40 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 142 pmole/min/µg. A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C. CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl 2 , 10 mM MgCl 2 , 2 mM DTT, 5 μM ATP) using a beta-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected. |
| SwissProt/Accession | Q8TCT0 |
| Gene | CERK |
| Residue | 1 to 537 |
| Sequence | MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGAPGADAC SVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWA QVTFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIY ERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMF SEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVG TSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDS EKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAG CFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCAC RRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFV EVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWN CDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS |
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