All lanes : Anti-c-Fos (phospho T232) antibody ( ab43175 ) at 1 µg/ml Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml) Lane 4 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM) Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml Lane 7 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml) with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml Lane 8 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM) with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml Lane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated with Human c-Fos peptide ( ab60297