SDS-PAGE Analysis of ab91577 stained with Coomassie Blue. Lane 1: MW Marker Lane 2: 0.5 µg purified Calreticulin Lane 3: 1 µg purified Calreticulin Lane 4: 2 µg purified Calreticulin Lane 5: 5 µg purified Calreticulin
All lanes : Human Calreticulin full length protein (ab91577) at 1/1000 dilution Lane 1 : MW Marker Lane 2 : 50ng Calreticulin Lane 3 : 100ng Calreticulin
Anti-Calreticulin antibody - ER Marker ( ab39818 ) at 1 µg/ml + Human Calreticulin full length protein (ab91577) at 0.01 µg Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) ( ab65484 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 30 seconds