Inhibition of TRAIL-mediated lysis. ab157352 exerts its inhibitory activity in a concentration range of 50-100µg/ml. Method: Human Jurkat lymphoma cells (50,000 cells in 100µl DMEM medium containing 5% fetal calf serum) were incubated with 0.1µg/ml soluble TRAIL plus 2µg/ml enhancer and increasing concentrations of ab157352 in a 96-well plate for 16 hours at 37°C. Concentrations of ab157352 required to inhibit may vary depending on the cell type studied and on the concentration of sTRAIL used to kill cells. Cell viability was determined using a MTT-based cell proliferation assay kit.