Recombinant Human MAdCAM-1 Fc Chimera Protein, CF

• Supplier: R&D Systems
• Catalog: 6056-MC
• Category: Protein
• Prices: $229.00
• Sizes: 50 µg
• Species Reactivities: Human
• Nature: Recombinant
• Source: Mouse myeloma cell line, NS0-derived Human MAdCAM-1 Met1-Gln333 Accession # AAY82472 IEGRMD Human IgG 1 (Pro100-Lys333) N-terminus C-terminus
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 10 4 cells/well are added to recombinant human MAdCAM-1/Fc Chimera coated plates (2.5 µg/mL with 100 µL/well), approximately 50%-70% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl 2 . Optimal dilutions should be determined by each laboratory for each application.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• SwissProt/Accession: AAY82472
• Gene: MADCAM1
• Sequence: Val23 & Glu29

Product References

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