| Name | Recombinant Human Fibulin 5 Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 3095-FB |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 25 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Chinese Hamster Ovary cell line, CHO-derived Gln24-Phe448, with a C-terminal 6-His tag |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Bioactivity | Measured by the ability of the immobilized protein to enhance the adhesion of HUVEC human umbilical vein endothelial cells. When 5 x 10 4 cells per well are added to rhFibulin-5 coated plate, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. ED 50 for this effect is typically 0.1-0.4 μg/mL. Optimal dilutions should be determined by each laboratory for each application. |
| Endotoxin | <0.10 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | NP_006320 |
| Gene | FBLN5 |
| Sequence | No results obtained: Gln24 predicted |
| Supplier Page | Shop |
Vierkotten S, Muether PS, Fauser S. PLoS One. 2011;6(8):e22959.
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J. Nature. 2012 Jul 26;487(7408):505-9.