| Name | Recombinant Human Frizzled-8 Fc Chimera Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 6129-FZ |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 50 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Chinese Hamster Ovary cell line, CHO-derived Human Frizzled-8 (Ala25-Pro172) Accession # Q9H461 IEGRMD Human IgG 1 (Pro100-Lys330) N-terminus C-terminus |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Bioactivity | Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED 50 for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN ) Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated recombinant mouse (rm) Wnt-3a or rmWnt-5a at 50-100 ng/mL and Recombinant Human (rh) Frizzled-8 Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-8 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 7-28 ng/mL. Optimal concentrations should be determined by each laboratory for each application. |
| Endotoxin | <0.10 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | Q9H461 |
| Gene | FZD8 |
| Sequence | Ala25 & Ala28 |
| Supplier Page | Shop |
Jackson H, Granger D, Jones G, Anderson L, Friel S, Rycroft D, Fieles W, Tunstead J, Steward M, Wattam T, Walker A, Griggs J, Al-Hajj M, Shelton C. Mol Cancer Res. 2016 Sep;14(9):859-68.
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J. Nature. 2012 Jul 26;487(7408):505-9.