Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF

• Supplier: R&D Systems
• Catalog: 8078-GP
• Category: Protein
• Prices: $329.00
• Sizes: 50 µg
• Species Reactivities: Human
• Nature: Recombinant
• Source: Chinese Hamster Ovary cell line, CHO-derived Human LGR-5/GPR49 (Gly22-Ile560) Accession # O75473 IEGRMD Human IgG 1 (Pro100-Lys330) N-terminus C-terminus
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Bioactivity: Measured by its binding ability in a functional ELISA. When Recombinant Human R‑Spondin 3 (Catalog # 3500-RS ) is coated at 0.5 μg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent K d <0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED 50 for this effect is 1.5-7.5 µg/mL in the presence of 20 ng/mL Recombinant Human R‑Spondin 2 (Catalog # 3266-RS ) and 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN ).
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• SwissProt/Accession: O75473
• Gene: LGR5
• Sequence: Gly22