| Name | Recombinant Human Thrombospondin-4 Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 2390-TH |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 50 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Chinese Hamster Ovary cell line, CHO-derived Ala22-Asn961, with a C-terminal 10-His tag |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. Recombinant Human Thrombospondin‑4 (rhThrombospondin-4) is coated to 96 well plates at 10 µg/mL (100 µL/well) overnight then reduced with 20 mM DTT for 30 minutes. When 2 x 10 4 cells/well are added to rhThrombospondin‑4 coated plates, ≥50% will adhere after one hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application. |
| Endotoxin | <1.0 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | P35443 |
| Gene | THBS4 |
| Sequence | Ala22 |
| Supplier Page | Shop |