Recombinant Human ENPP-7/Alk-SMase Protein, CF

Name Recombinant Human ENPP-7/Alk-SMase Protein, CF
Supplier R&D Systems
Catalog 4924-EN
Category Protein
Prices $329.00
Sizes 10 µg
Species Reactivities Human
Nature Recombinant
Source Mouse myeloma cell line, NS0-derived Ala22-Ser439, with a C-terminal 10-His tag
Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Bioactivity Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL (Catalog # 2909-AP ) and the phosphate is detected/measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >14,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com
Endotoxin <1.0 EU per 1 μg of the protein by the LAL method.
SwissProt/Accession NP_848638
Gene ENPP7
Sequence Ala22
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