Standard curve for Chromogranin A (Analyte: ab85486 ); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab36997 at 1µg/ml and Detector Antibody ab17064 at 0.5µg/ml. Concentration of ab17064 may vary from lot to lot; please use this curve as guideline.
12% SDS-PAGE analysis of 3µg of ab85486
Anti-Chromogranin A antibody ( ab15160 ) at 1/100 dilution + Human Chromogranin A full length protein (ab85486) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 30 seconds