Escherichia coli



Anti-2 Cys Peroxiredoxin antibody [6E5] - ab16765 from Abcam

Prices $380.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E5
ICC/IF image of ab16765 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16765, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-7 Alpha Hydroxysteroid Dehydrogenase antibody - ab54197 from Abcam

Prices $359.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-Acetate Kinase antibody - ab182938 from Abcam

Prices $370.00
Sizes 10 mg
Host Rabbit
Clonality Polyclonal

Anti-Ada protein antibody [ADA-1] - ab18104 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ADA-1

Anti-Alkaline Phosphatase antibody - ab354 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.See Abreview

Anti-Anaerobic ribonucleoside-triphosphate reductase antibody (Biotin) - ab193405 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Anaerobic ribonucleoside-triphosphate reductase antibody (FITC) - ab193429 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Asparaginase antibody - ab135225 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab135225 staining Asparaginase in Mice which had been injected, by iv, with Asparaginase or solvent and sacrificed 16 hours after injection. Tissues were stained for presence of Asparaginase by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked (2% Normal Goat serum and 2% BSA) for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 1 hours at 20°C. A Biotin-conjugated Goat anti-rabbit  monoclonal (1/1000) was used as the secondary antibody.See Abreview

Anti-Asparaginase antibody (Biotin) - ab34616 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab34616 staining Asparaginase in Mice treated with E.coli Asparaginase or mock treated mice were sacrificed 16 hours after injection. Tissue was stained for Asparaginase uptake by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2% Normal Goat serum + 2% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 1 hours at 20°C.See Abreview

Anti-Asparaginase antibody (Biotin) - ab20646 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-ATP-dependent Clp protease adapter protein ClpS antibody - ab193643 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ATP-dependent Clp protease adapter protein ClpS antibody (ab193643) at 2 µg/ml + DH5a lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilution

Anti-ATP-dependent Clp protease adapter protein ClpS antibody (Biotin) - ab193648 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ATP-dependent Clp protease adapter protein ClpS antibody (FITC) - ab193632 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ATP-dependent Clp protease adapter protein ClpS antibody (HRP) - ab193617 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ATPB antibody [7E3F2] - ab110280 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7E3F2
All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/mlLane 1 : Isolated mitochondria from Human heart at 15 µgLane 2 : Isolated mitochondria from cow heart at 6 µgLane 3 : Isolated mitochondria from rat heart at 30 µgLane 4 : Isolated mitochondria from mouse heart at 30 µgLane 5 : Isolated mitochondria from HepG2 cells at 30 µg

Anti-bacterial Alkaline Phosphatase antibody [BAP-77] - ab49793 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone BAP-77

Anti-beta Galactosidase antibody - ab9361 from Abcam

Host Chicken
Clonality Polyclonal
ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).See Abreview

Anti-beta Galactosidase antibody - ab616 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-beta Galactosidase antibody (ab616) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells transfected with lacZ-overexpressing plasmid at 15 µgSecondaryHRP-conjugated Goat anti-rabbit at 1/5000 dilutiondeveloped using the ECL techniqueObserved band size : 120 kDa (why is the actual band size different from the predicted?)Additional bands at : 90-100 kDa. We are unsure as to the identity of these extra bands.Exposure time : 2 secondsImage courtesy of an anonymous Abreview.See Abreview

Anti-beta Galactosidase antibody - ab12081 from Abcam

Prices $382.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutionPerformed under reducing conditions.

Anti-beta Galactosidase antibody - ab107114 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
Anti-beta Galactosidase antibody (ab107114) at 1 µg/ml + B-galactosidase from E. coli at 10 µgSecondaryGoat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-beta Galactosidase antibody - ab106567 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-beta Galactosidase antibody (ab106567) at 1 µg/mlLane 1 : beta Galactosidase at 0.005 µgLane 2 : beta Galactosidase at 0.025 µg

Anti-beta Galactosidase antibody [2E9] - ab10074 from Abcam

Prices $368.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E9

Anti-beta Galactosidase antibody (Biotin) - ab6645 from Abcam

Prices $395.00
Sizes 1 mg
Host Rabbit
Clonality Polyclonal

Anti-beta Galactosidase antibody (Biotin) - ab182911 from Abcam

Prices $372.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal

Anti-beta Galactosidase antibody (HRP) - ab191357 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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