p53 signaling pathway



Apaf-1 (D7G4) Rabbit mAb - 8723 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7G4
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Apaf-1 siRNA I (+), using Apaf-1 (D7G4) Rabbit mAb #8723 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D7G4) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Apaf-1 (D5C3) Rabbit mAb - 8969 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5C3
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Apaf-1 siRNA I #6229 (+), using Apaf-1 (D5C3) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D5C3) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Apaf-1 (R205) Antibody - 5088 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from THP-1, ACHN, and 293 cells using Apaf-1 (R205) Antibody.

Apaf-1 Antibody - 4452 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat, PC12 and C2C12 cells, using Apaf-1 Antibody.

Apaf-1 (C-19) - sc-7231 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Apaf-1 (C-19): sc-7231. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of neuronal cells.

Apaf-1 (D-18) - sc-33870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Apaf-1 (D-18): sc-33870. Western blot analysis of Apaf-1 expression in HUV-EC-C (A), ECV304 (B), K-562 (C), human PBL (D) and WI 38 (E) whole cell lysates.

Apaf-1 (H-324) - sc-8339 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Apaf-1 (H-324): sc-8339. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.

Apaf-1 (K-20) - sc-26685 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Apaf-1 (K-20): sc-26685. Western blot analysis of Apaf-1 expression in PC-12 (A), C2C12 (B) and human PBL (C) whole cell lysates.

Apaf-1 (N-19) - sc-7232 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Apaf-1 (T-17) - sc-33871 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Apaf-1 (13F11) - sc-135625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

Apaf-1 (18H2) - sc-135624 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

Apaf-1 (2E12) - sc-135623 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

Apaf-1 (2E10) - sc-65890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Apaf-1 (2E10): sc-65890. Western blot analysis of expression in K-562 whole cell lysate.

Apaf-1 (5E11) - sc-65891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Apaf-1 (5E11): sc-65891. Western blot analysis of Apaf-1 expression in Jurkat whole cell lysate.

Apaf-1 (24) - sc-135836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Apaf-1 (24): sc-135836. Western blot analysis of Apaf-1 expression in human endothelial whole cell lysate.

Anti-APAF1 antibody - ab2001 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab2001 at 1µg/ml staining Apaf1 in human heart tissue by IHC 

Anti-APAF1 antibody - ab2000 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab2000 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2000, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-APAF1 antibody - ab2445 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-APAF1 antibody - ab53152 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with ab53152 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

Anti-APAF1 antibody - ab14228 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-APAF1 antibody - ab46899 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Human testis stained with ab46899 using the antibody at a dilution of 1:200

Anti-APAF1 antibody [2E10] - ab36822 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E10
Lane 1 : Anti-APAF1 antibody [2E10] (ab36822) at 0.25 µg/mlLane 2 : Anti-APAF1 antibody [2E10] (ab36822) at 0.5 µg/mlLane 1 : K562 cell lysateLane 2 : K562 cell lysate

Anti-APAF1 antibody [5E11] - ab36824 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5E11
ab36824 at 0.5µg/ml staining APAF1 in K562 cells by IHC

Anti-APAF1 antibody [E38] - ab32372 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E38
Anti-APAF1 antibody [E38] (ab32372) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
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