Anti-PIWIL4 antibody
| Name | Anti-PIWIL4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21869 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse PIWIL4 |
| Blocking Peptide | Mouse PIWIL4 peptide |
| Description | Rabbit Polyclonal |
| Gene | PIWIL4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/mlLanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/mlLane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/mlLane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab21869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab21869 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic - The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic
Keam SP, Young PE, McCorkindale AL, Dang TH, Clancy JL, Humphreys DT, Preiss T, Hutvagner G, Martin DI, Cropley JE, Suter CM. Nucleic Acids Res. 2014 Aug;42(14):8984-95.
TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and - TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and
Yabuta Y, Ohta H, Abe T, Kurimoto K, Chuma S, Saitou M. J Cell Biol. 2011 Mar 7;192(5):781-95.