Lanes 1 - 2 : Anti-PIWIL4 antibody ( ab21869 ) at 2 µg/ml Lanes 3 - 4 : Anti-PIWIL4 antibody ( ab21869 ) at 1 µg/ml Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue ( ab4027 ) Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue ( ab4027 ) with Mouse PIWIL4 peptide (ab23535) at 1 µg/ml Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue ( ab4027 ) Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue ( ab4027 ) with Mouse PIWIL4 peptide (ab23535) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Rabbit IgG secondary antibody ( ab28446 ) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 98 kDa Observed band size : 98 kDa See Abreview