All lanes : Anti-CAPS1 antibody (ab32787) at 1 µg/mlLane 1 : Brain (Rat) Tissue Lysate at 10 µgLane 2 : Brain (Mouse) Tissue Lysate at 10 µgLane 3 : Heart (Mouse) Tissue Lysate at 10 µgLane 4 : Brain (Rat) Tissue Lysate with Rat CAPS1 peptide (ab32786) at 1 µg/mlLane 5 : Brain (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/mlLane 6 : Heart (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/mlSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.