UBE2J1

Gene Symbol UBE2J1
Entrez Gene 51465
Alt Symbol CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, UBC6E, Ubc6p
Species Human
Gene Type protein-coding
Description ubiquitin-conjugating enzyme E2, J1
Other Description HSUBC6e|non-canonical ubiquitin-conjugating enzyme 1|ubiquitin-conjugating enzyme E2 J1|ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)|ubiquitin-conjugating enzyme E2, J1, U|yeast ubiquitin-conjugating enzyme UBC6 homolog E
Swissprots Q9UF10 Q9P011 Q5W0N4 A8K3F9 Q53F25 Q9NY66 Q9P0S0 Q9NQL3 Q9BZ32 Q9Y385
Accessions EAW48555 EAW48556 Q9Y385 AB075487 AF151039 AAF36125 AF151834 AAD34071 AF161502 AAF29117 AJ245898 CAB83212 AJ420479 AJ420522 AJ420578 AJ420600 AK223464 BAD97184 AK290574 BAF83263 AK300629 BAG62320 BC013973 AAH13973 BF058850 DQ895144 ABM86070 EU176317 ABW03768 U93243 AAF21505 XM_011535887 XP_011534189 XM_011535888 XP_011534190 NM_016021 NP_057105
Function Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). {ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000269|PubMed:12082160, ECO:0000269|PubMed:22607976}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:12082160}; Single-pass type IV membrane protein {ECO:0000269|PubMed:12082160}.
Top Pathways Ubiquitin mediated proteolysis, Protein processing in endoplasmic reticulum

UBE2J1 (B-6) - sc-377002 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBE2J1 (B-6): sc-377002. Immunofluorescence stain-ing of methanol-fixed HeLa cells showing cytoplasmic localization.

UBE2J1 (G-16) - sc-109122 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

UBE2J1 (H-104) - sc-292491 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
UBE2J1 (H-104): sc-292491. Western blot analysis of UBE2J1 expression in HeLa (A) and Caki-1 (B) whole cell lysates.

UBE2J1 (N-13) - sc-109123 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

UBE2J1 (L-23) - sc-134138 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
UBE2J1 (L-23): sc-134138. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing cytoplasmic and membrane localization.

UBE2J1 (18-Y) - sc-100624 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
UBE2J1 (18-Y): sc-100624. Western blot analysis of UBE2J1 expression in HeLa whole cell lysate.

Anti-UBC6e antibody - ab39104 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBC6e antibody (ab39104) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-UBC6e antibody - ab57122 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-UBC6e antibody [3E6] - ab131581 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E6
All lanes : Anti-UBC6e antibody [3E6] (ab131581) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY UBC6e cDNALysates/proteins at 5 µg per lane.

Anti-UBE2J1 Antibody (clone 1F6) - LS-C174075 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F6
IHC of paraffin-embedded Human pancreas tissue using anti-UBE2J1 mouse monoclonal antibody.

Anti-UBE2J1 Antibody (clone 3D1) - LS-C174081 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3D1
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2J1 mouse monoclonal antibody.

Anti-UBE2J1 Antibody (clone 3D5) - LS-C337794 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3D5
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-UBE2J1 Antibody (clone 3C11) - LS-C174070 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C11
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2J1 mouse monoclonal antibody.

Anti-UBE2J1 Antibody (clone 1C7) - LS-C174104 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C7
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2J1 mouse monoclonal antibody.

Anti-UBE2J1 Antibody (clone 3E6) - LS-C174071 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E6
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2J1 mouse monoclonal antibody.

Anti-UBE2J1 Antibody (N-Terminus) - LS-C60103 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Ube2j1 Antibody - Western Blot. Western blot of affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over-expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.

Anti-UBE2J1 Antibody (aa1-30, APC) - LS-C242356 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30, AP) - LS-C242357 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30, Biotin) - LS-C242358 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30, FITC) - LS-C242359 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30, PE) - LS-C242364 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30, Azide-free, HRP) - LS-C247176 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30) - LS-C326149 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2J1 Antibody (aa1-30) - LS-C160381 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
UBE2J1 Antibody western blot of human placenta tissue lysates (35 ug/lane). The UBE2J1 antibody detected the UBE2J1 protein (arrow).

Anti-UBE2J1 Antibody (clone 1H10) - LS-C174074 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2J1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2J1.
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