PRIM1

Gene Symbol PRIM1
Entrez Gene 5557
Alt Symbol p49
Species Human
Gene Type protein-coding
Description primase, DNA, polypeptide 1 (49kDa)
Other Description DNA primase 1|DNA primase 49 kDa subunit|DNA primase small subunit|DNA primase subunit 48|primase p49 subunit|primase polypeptide 1, 49kDa
Swissprots P49642
Accessions AAC51726 P49642 AA761682 AK223391 BAD97111 AK314866 BAG37381 BC005266 AAH05266 BU167590 CD686660 EU176421 ABW03872 EU176762 ABW03563 X74330 CAA52377 NM_000946 NP_000937
Function DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
Top Pathways DNA replication, Purine metabolism, Pyrimidine metabolism

p48 Primase (8G10) Rat mAb - 4725 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rat
Clonality Monoclonal
Clone 8G10
Western blot analysis of extracts from various cell types using p48 Primase (8G10) Rat mAb.

PRIM1 (H-300) - sc-366482 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PRIM1 (H-300): sc-366482. Western blot analysis of PRIM1 expression in mouse kidney (A) and human liver (B) tissue extracts.

PRIM1 (C-12) - sc-162036 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PRIM1 (H-9) - sc-390265 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PRIM1 (H-9): sc-390265. Western blot analysis of PRIM1 expression in SP2/0 (A) and Hep G2 (B) whole cell lysates and human liver tissue extract (C).

PRIM1 (K-25) - sc-133925 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PRIM1 (K-25): sc-133925. Western blot analysis of PRIM1 expression in K-562 whole cell lysate.

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody - ab72845 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845) at 1/500 dilutionLane 1 : Molecular weight markersLane 2 : Whole cell lysate from serum starved HEK 293 cellsLane 3 : BSA negative control

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody - ab23875 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab23875)Lane 1 : Lysates prepared from HepG2 cells left unstimulated with 3% BSA-TBST buffer and no peptideLane 2 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and no peptideLane 3 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and the non-phosphopeptide corresponding to the immunogenLane 4 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and a generic phospho-threonine-containing peptideLane 5 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and the phosphopeptide immunogenLane 6 : Lysates prepared from HepG2 cells stimulated with Metformin and treated with lambda phosphatase with 3% BSA-TBST buffer Secondarygoat F(ab’)2 anti rabbit IgG HRP conjugate

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - ab133448 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5683
Overlay histogram showing HeLa cells stained with ab133448 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133448, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PRIM1 antibody - ab113412 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
All lanes : Anti-PRIM1 antibody (ab113412) at 0.00999999977648 µg/mlLane 1 : Human liver lysateLane 2 : Mouse liver lysateLysates/proteins at 35 µg per lane.

Anti-PRIM1 antibody - ab197120 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRIM1 antibody (ab197120) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysate

Anti-PRIM1 antibody - ab137917 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PRIM1 antibody (ab137917) at 1/500 dilution + K562 cell lysate at 30 µg

Anti-PRIM1 Antibody - LS-C36172 from LifeSpan Bioscience

Prices $415.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PRIM1 Antibody (C-Terminus) - LS-C135186 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PRIM1 antibody LS-C135186 Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml.

Anti-PRIM1 Antibody (aa371-420) - LS-C119913 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from COLO/HeLa cells, using PRIM1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-PRIM1 Antibody (aa24-73) - LS-C80787 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
K562 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.

Anti-PRIM1 Antibody (Lys413) - LS-C177826 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of PRIM1 (K413) pAb in paraffin-embedded human breast carcinoma tissue.

Anti-PRIM1 Antibody (C-Terminus) - LS-C139744 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
PRIM1 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PRIM1 Antibody (aa364-413) - LS-C200523 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from K562 cells using PRIM1 antibody.

DNA Primase small subunit Antibody - NBP1-58184 from Novus Biologicals

Prices $299.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DNA Primase small subunit Antibody [NBP1-58184] - Titration: 1.25ug/ml Positive Control: K562 cell lysate.

DNA Primase small subunit Antibody - NBP1-51357 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: DNA Primase small subunit Antibody [NBP1-51357] - Western blot (WB) analysis of PRIM1 (K413) pAb in extracts from Raw264.7 cells,treated with LPS.

DNA Primase small subunit Antibody - NBP1-90897 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: DNA Primase small subunit Antibody [NBP1-90897] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Anti-PRIM1, C-Terminal antibody produced in rabbit - SAB4502327 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PRIM1, C-Terminal: <B>Cat. No. SAB4502327</B>: Immunoblotting analysis of extracts from COLO/HeLa cells, using Anti-PRIM1, C-Terminal antibody. The lane on the left was treated with the Anti-PRIM1, C-Terminal antibody. The lane on the right (negative control) was treated with both Anti-PRIM1, C-Terminal antibody and the synthesized immunogen peptide.

Anti-PRIM1 antibody produced in rabbit - HPA040010 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PRIM1: <B>Cat. No. HPA040010</B>: Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

PRIM1 antibody - N-terminal region (ARP46013_T100) - ARP46013_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Prim1-rat-Antibody-C-Term - AF3467a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF3467a (0.01 µg/ml) staining of Human (A) and Mouse (B) Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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