AP3D1

Gene Symbol AP3D1
Entrez Gene 8943
Alt Symbol ADTD, hBLVR
Species Human
Gene Type protein-coding
Description adaptor-related protein complex 3, delta 1 subunit
Other Description AP-3 complex delta subunit, partial CDS|AP-3 complex subunit delta|AP-3 complex subunit delta-1|adapter-related protein complex 3 subunit delta-1|adaptin, delta|adaptor-related protein complex 3 subunit delta-1|delta adaptin|delta-adaptin, partial CDS|subunit of putative vesicle coat adaptor complex AP-3
Swissprots Q96G11 O00202 Q9H3C6 O75262 O14617 Q59HF5
Accessions AAC25623 AAC27670 AAC34212 AAC34214 EAW69412 EAW69416 O14617 AB208804 BAD92041 AF002163 AAC51761 AF130042 AAG35473 AK094460 BAG52868 AK128692 AK310099 AK315899 BAF98790 AY954498 BC005142 AAH05142 BC010065 AAH10065 BC022837 BM982261 BQ227203 JF432332 ADZ15549 U91930 AAD03777 XM_006722932 XP_006722995 NM_001077523 NM_001261826 NP_001248755 NM_003938 NP_003929
Function Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Subcellular Location Cytoplasm {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
Tissue Specificity Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis. {ECO:0000269|PubMed:9151686}.
Top Pathways Lysosome

AP-3δ (C-17) - sc-46768 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AP-3δ (C-17): sc-46768. Western blot analysis of AP-3δ isoform expression in IMR-32 whole cell lysate.

AP-3δ (N-18) - sc-46772 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AP-3δ (N-18): sc-46772. Western blot analysis of AP-3δ isoform expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

AP-3δ1 (K-20) - sc-49154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AP-3δ1 (N-15) - sc-49155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AP-3δ (18) - sc-136277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
AP-3δ (18): sc-136277. Western blot analysis of AP-3δ expression in HeLa whole cell lysate.

Anti-AP3 antibody - ab85075 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-AP3 antibody (ab85075) at 1/500 dilution + HeLa cell lysate

Anti-AP3 antibody - ab90520 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ab90520, at a dilution of 1/100, staining AP3 in the cytoplasm of formalin fixed, paraffin embedded Human fetal skeletal muscle tissue by Immunohistochemistry.

Anti-AP3 antibody - ab3705 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-AP3D1 / Adaptin Delta Antibody (Internal) - LS-C137156 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AP3D1 / Adaptin Delta Antibody - LS-C136880 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AP3D1 / Adaptin Delta Antibody (aa686-698) IHC-plus™ - LS-B345 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-AP3D1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B345 concentration 5 ug/ml.

AP3D1 Antibody - NBP2-27158 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: AP3D1 Antibody [NBP2-27158] - analysis using AP3 Delta antibody. HeLa lysate in the 1) absence and 2) presence of immunizing peptide, probed with AP3 Delta antibody at 2 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

AP3D1 Antibody - NBP1-02453 from Novus Biologicals

Prices $329.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Western Blot: AP3D1 Antibody [NBP1-02453] - Western blot using Affinity Purified anti-AP3D1 antibody shows detection of a 130-kD band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.

Anti-AP3D1 antibody produced in goat - SAB2900325 from Sigma-Aldrich

Prices $464.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AP3D1: <B>Cat. No.SAB2900325</B>: Lung, respiratory epithelium: Formalin-Fixed Paraffin-Embedded (FFPE).

Ap3D1 antibody - GTX23705 from GeneTex

Prices $389.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
Anti-Ap3D1 antibody used in Western blot. GTX23705

Anti -AP3D1 (D-adaptin 3, AP-3 complex delta subunit and D-adaptin) - 168027 from USBiological Life Science

Host Goat
Clonality Polyclonal

AP3D1 antibody - 70R-15752 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

AP3D1 Antibody (OALA03346) - OALA03346 from Aviva Systems Biology

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

AP3D1 Antibody - abx038276 from Abbexa

Host Rabbit
Clonality Polyclonal

AP3 complex subunit delta-1 antibody - 16454-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
HeLa cells were subjected to SDS PAGE followed by western blot with 16454-1-AP(AP3D1 antibody) at dilution of 1:300

AP3 complex subunit delta-1 / AP3D1 (686-698) antibody - AP07559PU-N from Acris Antibodies

Prices $430.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
AP07559PU-N AP3D1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Lung, Respiratory Epithelium at 2.5 µg/ml

AP3 complex subunit delta-1 (686-698) antibody - AP09210PU-N from Acris Antibodies

Prices $420.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western blot using Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A  chemiluminescence system was used for signal detection (Roche) using a 30-sec exposure time.

AP3 complex subunit delta-1 antibody - H00008943-B01P from Acris Antibodies

Prices $1,530.00
Sizes 500 µg
Host Mouse
Clonality Polyclonal

AP3 complex subunit delta-1 antibody - PAB9965 from Acris Antibodies

Prices $580.00
Sizes 100 µg
Clonality Polyclonal
Western blot using AP3D1 polyclonal antibody ( Cat # PAB9965 ) shows detection of a 130 kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specificbinding. Approximately 20 µg of lysate was run ona SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP3D1 polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-GoatIgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection using a 30-sec exposure time.
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