Gene Symbol | ARID5B |
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Entrez Gene | 84159 |
Alt Symbol | DESRT, MRF-2, MRF2 |
Species | Human |
Gene Type | protein-coding |
Description | AT rich interactive domain 5B (MRF1-like) |
Other Description | ARID domain-containing protein 5B|AT-rich interactive domain 5B (MRF1-like)|AT-rich interactive domain-containing protein 5B|MRF1-like protein|modulator recognition factor 2 (MRF2) |
Swissprots | Q9H786 Q14865 Q32Q59 Q05DG6 B4DLB3 Q8N421 Q5VST4 Q6NZ42 Q7Z3M4 |
Accessions | EAW54226 EAW54227 EAW54228 Q14865 AI149153 AK024803 BAB15012 AK123882 AK296921 BAG59475 AL049471 BC015120 AAH15120 BC017404 BC036831 AAH36831 BC056680 BC066345 AAH66345 BC107800 AAI07801 BC152736 AAI52737 BC156570 AAI56571 BG615173 BX352258 BX472565 BX537690 CAD97814 BX641020 CAE46013 BX648835 DB014195 LN607831 CEF49518 M73837 AAA59870 XM_011540262 XP_011538564 NM_001244638 NP_001231567 NM_032199 NP_115575 |
Function | Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. {ECO:0000269|PubMed:21532585}. |
Subcellular Location | Nucleus {ECO:0000255|PROSITE- ProRule:PRU00355}. |
Tissue Specificity | Widely expressed, including in liver (at protein level). {ECO:0000269|PubMed:21532585}. |
Mrf-2 CRISPR/Cas9 KO Plasmid (h) - sc-409123 from Santa Cruz Biotechnology
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Mrf-2 HDR Plasmid (h) - sc-409123-HDR from Santa Cruz Biotechnology
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Mrf-2 Double Nickase Plasmid (h) - sc-409123-NIC from Santa Cruz Biotechnology
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Mrf-2 Double Nickase Plasmid (h2) - sc-409123-NIC-2 from Santa Cruz Biotechnology
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Mrf-2 CRISPR Activation Plasmid (h) - sc-409123-ACT from Santa Cruz Biotechnology
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Mrf-2 CRISPR Activation Plasmid (h2) - sc-409123-ACT-2 from Santa Cruz Biotechnology
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Mrf-2 Lentiviral Activation Particles (h) - sc-409123-LAC from Santa Cruz Biotechnology
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Mrf-2 Lentiviral Activation Particles (h2) - sc-409123-LAC-2 from Santa Cruz Biotechnology
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Mrf-2 siRNA (h) - sc-90532 from Santa Cruz Biotechnology
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Mrf-2 shRNA Plasmid (h) - sc-90532-SH from Santa Cruz Biotechnology
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Mrf-2 shRNA (h) Lentiviral Particles - sc-90532-V from Santa Cruz Biotechnology
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ARID5B RNAi - H00084159-R01 from Novus Biologicals
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MISSION® esiRNA - EHU065951 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3474 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3474 from Sigma-Aldrich
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ARID5B siRNA (Human) - CRJ3327 from Cohesion Biosciences
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ARID5B - MBS8240418 from MyBioSource
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shRNA set against Human ARID5B (NM_032199.2) - SHH240034 from Creative biogene
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Human ARID5B siRNA - orb273828 from Biorbyt
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ARID5B shRNA Plasmid (Human) - CHJ3327 from Cohesion Biosciences
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