CARF

Gene Symbol	CARF
Entrez Gene	79800
Alt Symbol	ALS2CR8, NYD-SP24
Species	Human
Gene Type	protein-coding
Description	calcium responsive transcription factor
Other Description	amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8\|amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein\|calcium-response factor\|calcium-responsive transcription factor\|testis development protein NYD-SP24
Swissprots	Q8N187 B4E1W7 Q96Q39 G3V1K7 Q96Q38 Q96J78 Q9H712 Q8WXC0 Q8ND29
Accessions	AAY24288 EAW70320 EAW70321 EAW70322 EAW70323 EAW70324 Q8N187 AB053309 BAB69018 AB053310 BAB69019 AF454946 AAL62330 AK022737 AK025232 BAB15088 AK225218 AK295499 BAG58419 AK295703 BAG58550 AK299366 BAG61358 AK304014 BAG64929 AK308171 AK310640 AK316432 BAH14803 AL834188 CAD38882 AV751848 AY032876 AAK66819 BC021999 BC033777 AAH33777 CK825814 DA405623 DB094349 DQ891348 ABM82274 DQ894528 ABM85454 XM_005246858 XP_005246915 XM_005246859 XP_005246916 XM_005246860 XP_005246917 XM_005246863 XP_005246920 XM_005246864 XP_005246921 XM_006712757 XP_006712820 XM_006712759 XP_006712822 XM_006712760 XP_006712823 XM_011511867 XP_011510169 XM_011511868 XP_011510170 XM_011511869 XP_011510171 NM_001104586 NP_001098056 NM_001282910 NP_001269839 NM_001282911 NP_001269840 NM_001282912 NP_001269841 NM_024744 NP_079020
Function	Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence. {ECO:0000269\|PubMed:11832226, ECO:0000269\|PubMed:22174809}.
Subcellular Location	Nucleus {ECO:0000250}.

CARF (S-20) - sc-74909 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

CARF (S-20): sc-74909. Western blot analysis of CARF expression in Jurkat (A) and CCRF-CEM (B) nuclear extracts.

CARF (P-18) - sc-74908 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

CARF (P-18): sc-74908. Western blot analysis of CARF expression in non-transfected: sc-117752 (A) and human CARF transfected: sc-159702 (B) 293T whole cell lysates.

CARF (H-92) - sc-368848 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

CARF (14.4) - sc-101206 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

CARF (14.4): sc-101206. Western blot analysis of CARF expression in non-transfected 293T: sc-117752 (A), human CARF transfected 293T: sc-114989 (B) and HeLa (C) whole cell lysates.

Anti-ALS2CR8 antibody - ab92531 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ALS2CR8 antibody (ab92531) at 1/500 dilutionLane 1 : Extract from HuvEc cells (without immunising peptide)Lane 2 : Extract from HuvEc cells (with immunising peptide)Lysates/proteins at 5 µg per lane.

Anti-ALS2CR8 antibody - ab197813 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ALS2CR8 antibody (ab197813) at 1/300 dilutionLane 1 : Human testis tissue lysateLane 2 : K562 cell lysateLysates/proteins at 40 µg per lane.Secondarysecondary antibody at 1/8000 dilution

Anti-ALS2CR8 antibody - ab69480 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-ALS2CR8 antibody (ab69480) at 1/500 dilutionLane 1 : ALS2CR8 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-ALS2CR8 antibody [EPR13712] - ab180142 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13712

Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling ALS2CR8 with ab180142 at 1/90. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG

Anti-ALS2CR8 antibody - N-terminal - ab138624 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ALS2CR8 antibody - N-terminal (ab138624) at 1/100 dilution + K562 cell lysate at 35 µg

Anti-CARF / ALS2CR8 Antibody (aa131-158, APC) - LS-C232571 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa131-158, AP) - LS-C232570 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa131-158, Biotin) - LS-C232572 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa131-158, PE) - LS-C232574 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa131-158, FITC) - LS-C232573 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa131-158, Azide-free, HRP) - LS-C249405 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa311-360) - LS-C119724 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human lung carcinoma tissue, using ALS2CR8 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-CARF / ALS2CR8 Antibody (aa132-158) - LS-C161045 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

ALS2CR8 Antibody western blot of K562 cell line lysates (35 ug/lane). The ALS2CR8 antibody detected the ALS2CR8 protein (arrow).

Anti-CARF / ALS2CR8 Antibody - LS-C306224 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa225-275) - LS-C290511 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human CARF by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CARF antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-CARF / ALS2CR8 Antibody (aa131-158) - LS-C329208 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CARF / ALS2CR8 Antibody (aa1-50) - LS-C290514 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human and Mouse CARF by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), Jurkat (J; 50 ug), and NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CARF antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-CARF / ALS2CR8 Antibody (clone 2A3) - LS-C133816 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A3

Immunoperoxidase of monoclonal antibody to ALS2CR8 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ug/ml]

ALS2CR8 Antibody (2A3) - H00079800-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A3

Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - ALS2CR8 monoclonal antibody (M01), clone 2A3 Analysis of ALS2CR8 expression in Hela S3 NE.

ALS2CR8 Antibody - NBP1-86627 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: ALS2CR8 Antibody [NBP1-86627] - Immunofluorescent staining of human cell line U-251MG shows positivity in nucleoli & cytoplasm.

ALS2CR8 Antibody - H00079800-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: ALS2CR8 Antibody [H00079800-B01P] - Analysis of ALS2CR8 expression in transfected 293T cell line by ALS2CR8 polyclonal antibody. Lane 1: ALS2CR8 transfected lysate(78.98 KDa). Lane 2: Non-transfected lysate.

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