DDX4

Gene Symbol DDX4
Entrez Gene 54514
Alt Symbol VASA
Species Human
Gene Type protein-coding
Description DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Other Description DEAD box protein 4|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|probable ATP-dependent RNA helicase DDX4|vasa homolog
Swissprots A8K8Q2 D6RDK4 Q9NYB1 Q5M7Z3 Q9NQI0 E9PCD8 Q86VX0 B3KSF4 Q9NT92
Accessions CBH19257 EAW54929 Q9NQI0 AF262962 AAF72705 AK093439 BAG52716 AK292417 BAF85106 AK308300 AL137462 CAB70750 AY004154 AAF86585 BC030638 BC047455 AAH47455 BC088362 AAH88362 BI559479 DB022413 DB051674 DB457419 DB508256 XM_005248545 XP_005248602 XM_005248547 XP_005248604 XM_011543495 XP_011541797 XM_011543496 XP_011541798 XM_011543497 XP_011541799 NM_019039 NM_001142549 NP_001136021 NM_001166533 NP_001160005 NM_001166534 NP_001160006 NM_024415 NP_077726
Function May play a role in germ cell development. May play a role in sperm motility. {ECO:0000269|PubMed:10920202, ECO:0000269|PubMed:21034600}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:10920202, ECO:0000269|PubMed:21421998}. Cytoplasm, perinuclear region {ECO:0000250}.
Tissue Specificity Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. {ECO:0000269|PubMed:10920202}.

DDX4 (D10C5) Rabbit mAb - 8761 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10C5
Western blot analysis of extracts from mouse testes and brain using DDX4 (D10C5) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

DDX4 (2F9H5) Mouse mAb - 5555 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2F9H5
Western blot analysis of extract from human testis tissue using DDX4 (2F9H5) Mouse mAb.

DDX4 Antibody - 8227 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from human and mouse testes using DDX4 Antibody.

Vasa (C-2) - sc-514249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vasa (C-2): sc-514249. Western blot analysis of Vasa expression in Schneiders <i>Drosophila</i> Line 2 whole cell lysate.

VASA (L18Z) - sc-80427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
VASA (L18Z): sc-80427. Western blot analysis of VASA expression in non-transfected: sc-117752 (A) and human VASA transfected: sc-111520 (B) 293T whole cell lysates.

VASA (H-80) - sc-67185 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VASA (H-80): sc-67185. Western blot analysis of VASA expression in non-transfected 293T: sc-117752 (A) and human VASA transfected 293T: sc-111520 (B) whole cell lysates and rat testis tissue extract (C).

VASA (K-14) - sc-48705 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VASA (N-14) - sc-48707 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VASA (N-14): sc-48707. Western blot analysis of VASA expression in rat testis tissue extract.

Anti-DDX4 / MVH antibody - ab119322 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDX4 / MVH antibody (ab119322) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : 3T3 cell lysate

Anti-DDX4 / MVH antibody - ab130721 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DDX4 / MVH antibody (ab130721) at 1/100 dilution + WiDr cell lysate at 35 µg

Anti-DDX4 / MVH antibody - ab134030 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue labelling DDX4/MVH with ab134030 at 5 µg/ml. Followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-DDX4 / MVH antibody [2F9H5] - ab130184 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2F9H5
Anti-DDX4 / MVH antibody [2F9H5] (ab130184) at 1/500 dilution + DDX4 / MVH-hIgGFc transfected HEK293 cell lysate

Anti-DDX4 / MVH antibody [EPR3849] - ab108392 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3849
All lanes : Anti-DDX4 / MVH antibody [EPR3849] (ab108392) at 1/10000 dilutionLane 1 : Human testis lysateLane 2 : Human ovary cancer lysateLane 3 : K562 cell lysateLane 4 : Seminoma cell lysateLane 5 : HeLa cell lysateLane 6 : NCCIT cell lysateLane 7 : LNCaP cell lysateLysates/proteins at 10 µg per lane.

Anti-DDX4 / MVH antibody [mAbcam27591] - ab27591 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam27591
ICC/IF image of ab27591 stained mES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27591, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DDX4 / MVH antibody [mAbcam27591] (Alexa Fluor® 647) - ab196708 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam27591
ab196708 staining DDX4 / MVH in mouse embryonic stem cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196708 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in mouse embryonic stem cells fixed with 4% formaldehyde (10 min).

Anti-DDX4 / MVH antibody [mAbcam27591] (HRP) - ab196709 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam27591
Anti-DDX4 / MVH antibody [mAbcam27591] (HRP) (ab196709) at 1/8000 dilution + Testis (Mouse) Tissue Lysate at 20 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DDX4 / MVH antibody [MM0598-11G15] - ab89933 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0598-11G15

Anti-DDX4 / VASA Antibody (aa253-272) - LS-C313094 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
DDX4 / VASA antibody. IHC(F): Rat Intestine Tissue.

Anti-DDX4 / VASA Antibody (aa103-131, AP) - LS-C234501 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX4 / VASA Antibody (aa103-131, APC) - LS-C234503 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX4 / VASA Antibody (aa103-131, Biotin) - LS-C234504 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX4 / VASA Antibody (aa103-131, FITC) - LS-C246833 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX4 / VASA Antibody (Internal, clone EPR3849) - LS-C137764 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3849
Western blot analysis on (A) human testis, (B) human ovary cancer, (C) K562, (D) seminoma, (E) HeLa, (F) NCCIT and (G) LNCaP cell lysates using anti-DDX4 antibody.

Anti-DDX4 / VASA Antibody (aa103-131, PE) - LS-C246835 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX4 / VASA Antibody (aa103-131, Azide-free, HRP) - LS-C249813 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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