Gene Symbol | RASGRP2 |
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Entrez Gene | 10235 |
Alt Symbol | CALDAG-GEFI, CDC25L |
Species | Human |
Gene Type | protein-coding |
Description | RAS guanyl releasing protein 2 (calcium and DAG-regulated) |
Other Description | F25B3.3 kinase-like protein|RAS guanyl nucleotide-releasing protein 2|RAS guanyl-releasing protein 2|calcium and DAG-regulated guanine nucleotide exchange factor I|calcium and diacylglycerol-regulated guanine nucleotide exchange factor I|cdc25-like protein|guanine exchange factor MCG7|hCDC25L |
Swissprots | A6NDC7 Q9UL65 O00538 Q7LDG7 |
Accessions | CAI45372 EAW74281 EAW74282 EAW74283 Q7LDG7 AF043722 AAF07219 AF043723 AAF07220 AF081194 AAC79698 AK092852 AK092882 BAG52620 AK122694 BAG53672 AK124023 AK314637 BAG37199 BC110306 AAI10307 BC117151 AAI17152 DA286186 DB498812 HQ258466 ADR83220 U78170 AAD12741 Y12336 CAA73005 XM_005273707 XP_005273764 XM_011544718 XP_011543020 XM_011544719 XP_011543021 XM_011544720 XP_011543022 XM_011544721 XP_011543023 XM_011544722 XP_011543024 XM_011544723 XP_011543025 XM_011544724 XP_011543026 XM_011544725 XP_011543027 NM_001098670 NP_001092140 NM_001098671 NP_001092141 NM_153819 NP_722541 NM_005825 |
Function | Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. {ECO:0000269|PubMed:10918068, ECO:0000269|PubMed:14702343, ECO:0000269|PubMed:17576779, ECO:0000269|PubMed:17702895, ECO:0000269|PubMed:24958846}. |
Subcellular Location | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome {ECO:0000305}. Cell projection, ruffle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity). {ECO:0000250}. |
Tissue Specificity | Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney. {ECO:0000269|PubMed:10918068, ECO:0000269|PubMed:17576779, ECO:0000269|PubMed:17702895, ECO:0000269|PubMed:9341881, ECO:0000269|PubMed:9789079}. |
Top Pathways | MAPK signaling pathway, Chemokine signaling pathway, Rap1 signaling pathway |
RasGRP2 CRISPR/Cas9 KO Plasmid (h) - sc-408690 from Santa Cruz Biotechnology
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RasGRP2 HDR Plasmid (h) - sc-408690-HDR from Santa Cruz Biotechnology
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RasGRP2 Double Nickase Plasmid (h) - sc-408690-NIC from Santa Cruz Biotechnology
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RasGRP2 Double Nickase Plasmid (h2) - sc-408690-NIC-2 from Santa Cruz Biotechnology
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RasGRP2 CRISPR Activation Plasmid (h) - sc-408690-ACT from Santa Cruz Biotechnology
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RasGRP2 CRISPR Activation Plasmid (h2) - sc-408690-ACT-2 from Santa Cruz Biotechnology
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RasGRP2 Lentiviral Activation Particles (h) - sc-408690-LAC from Santa Cruz Biotechnology
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RasGRP2 Lentiviral Activation Particles (h2) - sc-408690-LAC-2 from Santa Cruz Biotechnology
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RasGRP2 siRNA (h) - sc-96904 from Santa Cruz Biotechnology
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RasGRP2 shRNA Plasmid (h) - sc-96904-SH from Santa Cruz Biotechnology
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RasGRP2 shRNA (h) Lentiviral Particles - sc-96904-V from Santa Cruz Biotechnology
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RASGRP2 RNAi - H00010235-R05 from Novus Biologicals
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RASGRP2 RNAi - H00010235-R06 from Novus Biologicals
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MISSION® esiRNA - EHU065451 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17985 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17985 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00914 from Sigma-Aldrich
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RASGRP2 siRNA (Human) - CRH6922 from Cohesion Biosciences
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RASGRP2 - MBS8205840 from MyBioSource
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shRNA set against Human RASGRP2 (NM_001098670.1) - SHH390036 from Creative biogene
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shRNA set against Human RASGRP2(NM_153819.1) - SHR166314 from Creative biogene
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Human RASGRP2 siRNA - orb267424 from Biorbyt
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RASGRP2 shRNA Plasmid (Human) - CHH6922 from Cohesion Biosciences
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RASGRP2 miRNA 3'UTR clone - MiUTR1H-08646 from Creative biogene
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RASGRP2 miRNA 3'UTR clone - MiUTR3H-07805 from Creative biogene
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