HMGN5

Gene Symbol	HMGN5
Entrez Gene	79366
Alt Symbol	NBP-45, NSBP1
Species	Human
Gene Type	protein-coding
Description	high mobility group nucleosome binding domain 5
Other Description	high mobility group nucleosome-binding domain-containing protein 5\|high-mobility group nucleosome binding domain 5\|nucleosomal binding protein 1\|nucleosome-binding protein 1
Swissprots	P82970 Q5JSL1
Accessions	AAK14063 EAW98582 P82970 AF250329 AAK14062 AK094058 AK315792 BAG38137 BC005342 AAH05342 DA745749 EU446988 ABZ92517 XM_006724688 XP_006724751 XM_006724689 XP_006724752 XM_006724690 XP_006724753 NM_030763 NP_110390
Function	Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. {ECO:0000250}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:11161810}. Note=Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin. {ECO:0000250}.
Tissue Specificity	Ubiquitously expressed. {ECO:0000269\|PubMed:11161810}.

NSBP1 (M-16) - sc-102042 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

NSBP1 (M-16): sc-102042. Western blot analysis of NSBP1 expression in Hep G2 whole cell lysate.

NSBP1 (N-13) - sc-132755 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-NSBP1 antibody - ab56031 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 antibody (ab56031) at 1 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-NSBP1 antibody - ab186001 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NSBP1 antibody (ab186001) at 0.1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-NSBP1 antibody - N-terminal - ab198205 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin embedded human prostrate cancer tissue labeling NSBP1 with ab198205 at a 1/100 dilution.

Anti-NSBP1 antibody - N-terminal - ab173082 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 antibody - N-terminal (ab173082) at 1/100 dilution + 293 cell line lysate at 35 µg

Anti-NSBP1 / HMGN5 Antibody (aa59-86) - LS-C322016 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa122-171) - LS-C30478 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa232-282) - LS-C290846 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human HMGN5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HMGN5 antibody used for IP at 6 ug/mg lysate. HMGN5 was also immunoprecipitated by rabbit anti-HMGN5 antibody BL15411. For blotting immunoprecipitated HMGN5, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-NSBP1 / HMGN5 Antibody (aa60-86, AP) - LS-C258667 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa60-86, APC) - LS-C258668 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa60-86, Biotin) - LS-C258669 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa60-86, FITC) - LS-C258670 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa60-86, Azide-free, HRP) - LS-C258671 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSBP1 / HMGN5 Antibody (aa60-86) - LS-C159853 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

HMGN5 Antibody western blot of 293 cell line lysates (35 ug/lane). The HMGN5 antibody detected the HMGN5 protein (arrow).

Anti-NSBP1 / HMGN5 Antibody (aa60-86, PE) - LS-C258672 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

NSBP1 Antibody - NBP1-87379 from Novus Biologicals

Prices	$129.00, $419.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: NSBP1 Antibody [NBP1-87379] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

NSBP1 Antibody - H00079366-B02 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: NSBP1 Antibody [H00079366-B02] - analysis of NSBP1 expression in transfected 293T cell line by NSBP1 MaxPab polyclonal antibody (B02). Lane1: NSBP1 transfected lysate (31.50 KDa). Lane2: Non-transfected lysate.

NSBP1 Polyclonal Antibody - bs-0700R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti NSBP1 Polyclonal Antibody, Unconjugated (bs-0700R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-HMGN5 antibody produced in rabbit - SAB4301006 from Sigma-Aldrich

Prices	$230.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGN5 antibody produced in rabbit - HPA000511 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-HMGN5: <B>Cat. No. HPA000511</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing nuclear staining of hepatocytes.

NSBP1 antibody - middle region (ARP37788_P050) - ARP37788_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

HMGN5-Antibody-N-term - AP17358a from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	80 µl, 400 µl
Host	Rabbit
Clonality	Polyclonal

HMGN5 Antibody (N-term) (Cat. #AP17358a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HMGN5 antibody detected the HMGN5 protein (arrow).

HMGN5 Antibody - N-terminal region (OAAB12789) - OAAB12789 from Aviva Systems Biology

Prices	$289.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

NSBP1 antibody - orb11169 from Biorbyt

Prices	$255.00, $340.00
Sizes	100 μg, 200 μg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse embryo tissue ( dilution at:1:200) using NSBP1 antibody

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